Tag: 16s

Concordance between culture, Molecular Culture and Illumina 16S rRNA gene amplicon sequencing of bone and ulcer bed biopsies in people with diabetic foot osteomyelitis #ActAgainstAmputation #Diabetes #diabeticfoot

Great work from our Dutch SALSAmigos Gramberg and coworkers led by Edgar JG Peters-- who was part of our original UT research unit. Background In clinical practice the diagnosis of diabetic foot osteomyelitis (DFO) relies on cultures of bone or ulcer bed (UB) biopsies, of which bone biopsy is reference standard. The slow growth or... Continue Reading →

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Metagenomics of Diabetic Foot Ulcer Undergoing Treatment with Total Contact Casting (TCC) @USC_vascular @USC @ALPSlimb @CosmosID

This initial look into what may be a routine survey of metagenomic data from week to week. Led by FASMA's Adam Isaac, CosmosID's (National Science Foundation Director Emerita) Prof. Rita Colwell and our team. Objective: Diabetic foot ulcers (DFUs) are characterised by the presence of many microbes, some of which may not be identified by traditional... Continue Reading →

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Metagenomics to Identify Pathogens in Diabetic Foot Ulcers and the Potential Impact for Clinical Care –

Diabetic foot ulcers are a growing problem and clinicians are limited by their reliance on conventional culture. Metagenomic sequencing technology provides an unparalleled viewpoint of the polymicrobial constituency of DFU. The microbiome techniques used to study the microbial constituency of DFU ma … — Read on pubmed.ncbi.nlm.nih.gov/34152440/ We are still in a bit of a... Continue Reading →

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