Tag: molecular microbiology

Toward non-invasive collection methods for sampling the microbiome of diabetic foot ulcers #Infection #Diabetes #ActAgainstAmputation #DiabeticFoot

Strong work from Bradbury and coworkers in the IWJ. Bottom line: Both swabs and tissues appeared to perform well for 16s sampling and sequencing. Identifying the microbiome within chronic diabetic foot ulcers is essential if effective antimicrobial therapies are to be administered. Using culture and 16S rRNA gene sequencing, the aim of this study was... Continue Reading →

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Concordance between culture, Molecular Culture and Illumina 16S rRNA gene amplicon sequencing of bone and ulcer bed biopsies in people with diabetic foot osteomyelitis #ActAgainstAmputation #Diabetes #diabeticfoot

Great work from our Dutch SALSAmigos Gramberg and coworkers led by Edgar JG Peters-- who was part of our original UT research unit. Background In clinical practice the diagnosis of diabetic foot osteomyelitis (DFO) relies on cultures of bone or ulcer bed (UB) biopsies, of which bone biopsy is reference standard. The slow growth or... Continue Reading →

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Probiotics and the gut-wound axis

Here is more cogent information to support the contention that we might consider adding bacteria rather than just removing it. To (again) use a military analogy, what one might describe as "clear, hold, and build" rather than just "clear". A Review of Immunomodulatory Reprogramming by Probiotics in Combating Chronic and Acute Diabetic Foot Ulcers (DFUs)... Continue Reading →

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Transcriptomic Fingerprint of Bacterial Infection in Lower Extremity Ulcers @ALPSLimb #ActAgainstAmputation

Important work from our Danish and Aussie colleagues Abstract Background and purpose: Clinicians and researchers utilize subjective, clinical classification systems to stratify lower extremity ulcer infections for treatment and research. The purpose of this study was to examine whether these clinical classifications are reflected in the ulcer's transcriptome. Methods: RNA-sequencing (RNA-seq) was performed on biopsies from clinically... Continue Reading →

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Metagenomics to Identify Pathogens in Diabetic Foot Ulcers and the Potential Impact for Clinical Care –

Diabetic foot ulcers are a growing problem and clinicians are limited by their reliance on conventional culture. Metagenomic sequencing technology provides an unparalleled viewpoint of the polymicrobial constituency of DFU. The microbiome techniques used to study the microbial constituency of DFU ma … — Read on pubmed.ncbi.nlm.nih.gov/34152440/ We are still in a bit of a... Continue Reading →

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