A Review of Genetic Engineering Biotechnologies for Enhanced Chronic Wound Healing

John W. Sessions,
David G. Armstrong,
Sandra Hope,
Brian D. Jensen

Abstract

Traditional methods for addressing chronic wounds focus on correcting dysfunction by controlling extracellular elements. This review highlights technologies that take a different approach – enhancing chronic wound healing by genetic modification to wound beds. Featured cutaneous transduction/transfection methods include viral modalities (i.e. adenoviruses, adeno-associated viruses, retroviruses, and lentiviruses) and conventional non-viral modalities (i.e. naked DNA injections, micro-seeding, liposomal reagents, particle bombardment, and electroporation). Also explored are emerging technologies, focusing on the exciting capabilities of wound diagnostics such as pyrosequencing as well as site-specific nuclease editing tools such as CRISPR-Cas9 used to both transiently and permanently genetically modify resident wound bed cells. Additionally, new non-viral transfection methods (i.e. conjugated nanoparticles,
multi-electrode arrays, and microfabricated needles and nanowires) are discussed that can potentially facilitate more efficient and safe transgene delivery to skin but also represent significant advances broadly to tissue regeneration research.screenshot 2017-01-08 at 2.25.35 PM.JPG

 

Source: A Review of Genetic Engineering Biotechnologies for Enhanced Chronic Wound Healing – Sessions – 2016 – Experimental Dermatology – Wiley Online Library

 

David G. Armstrong

Dedicated to amputation prevention, wound healing, diabetic foot, biotechnology and the intersection between medical devices and consumer electronics.

3 comments

  • in a few words, the researcher makes a statement of failure of current treatments and proposes a new way of doing things. it is still far from the reality of chronic injuries in the complication of diabetes but, it is a step forward.

  • Currently, the approach to managing chronic wounds is based on trying to control the environmental elements of the wound and surrounding tissues. Common standard of care approaches for wound care include the following: debridement (surgical or agent-based) of non-viable tissues, anti-inflammatories, antibiotics, moisture correction with wound dressings, and re-epithelialization and granulation of the wound bed by promotional adjuvants.[5-7] Unfortunately, these traditional methods for treatment do not always result in either complete or prompt wound closure. Chronic wounds are by their very nature complex because of the myriad of factors which can make each wound uniquely difficult to heal.[8-12]

    Exactly what I have practiced to cure the wound. Only my success comes from a different approach and the material to use and the surface that is processed is different
    Exactly what I have practiced to cure the wound. Only, my success comes from a different approach and the material to use and the surface that is treated is different .. the goal is to reach the whole body. Nprmal, since the complications of diabetes is a single disease. When you cut a foot, there is a rebound in the system and it is death that follows. you will never be able to treat just one organ in marginof the complete metabolism. A treatment for the whole body, the only way to get there, I have the way to do it and I have the product that can do it and I have the way to do it.
    Please hear me….

Leave a Reply