Dakin’s solution, also known as diluted sodium hypochlorite (0.5%), is an antiseptic used to kill germs and prevent bacterial growth in wounds.
It was first developed during World War I by Henry Drysdale Dakin and Alexis Carrel. Their collaboration arose from the urgent need to reduce the very high rates of wound infection, morbidity, and mortality among soldiers. At the time, carbolic acid (popularized by Lister and later Halstead) was widely used, but it was harsh and caustic. In contrast, Carrel-Dakin solution, given as an intermittent irrigation, proved much more wound-friendly — and remains in use today.
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Supplies Needed
• Sodium hypochlorite solution 5.25% (Clorox® or similar unscented household bleach)
Do not use scented or ultra-concentrated bleach products.
• Sodium bicarbonate (baking soda)
• Clean tap water
• Clean pan with lid
• Sterile measuring cup and spoons
Run through a dishwasher on the hottest setting for sterilization.
• Sterile jar with sterile lid
• Aluminum foil (to protect solution from light)
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Preparation Instructions
1. Wash your hands thoroughly with soap and water.
2. Gather all supplies listed above.
3. Measure 32 ounces (4 cups) of tap water and pour into the clean pan.
4. Boil water for 15 minutes with the lid on. Remove from heat.
5. Add ½ teaspoon of baking soda to the boiled water using a sterile spoon.
6. Following your doctor’s prescribed strength, add bleach according to the dilution chart (see below).
7. Pour the prepared solution into a sterile jar, seal with a sterile lid, and cover with aluminum foil to protect from light.
8. Label the jar clearly (see sample below).
Important: Discard any unused portion 48 hours after opening.
Unopened jars may be stored for up to one month.
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Dilution Chart for Dakin’s Solution
Use this chart to measure the correct amount of unscented household bleach (Clorox® 5.25%) to add to 32 ounces (4 cups) of water:
| Strength | Bleach (Clorox® 5.25%) | Water |
|---|---|---|
| Full Strength | 3 oz (95 ml) | 32 oz |
| ½ Strength | 1 Tbsp + 2 tsp | 32 oz |
| ¼ Strength | 1 Tbsp + ½ tsp | 32 oz |
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Sample Label
Dakin’s Solution
Dilution: _______________
Date/Time Prepared: _____
Discard After: ___________
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Precautions
• Keep out of the reach of children.
• If used as a mouth rinse, do not swallow.
• Do not use longer than 1 week, unless directed by your doctor.
• Do not use if allergic to any ingredients.
• Stop use if your condition worsens or if you develop a rash, irritation, or other reaction.
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Call Your Doctor If You Notice:
• Pain or burning sensation
• Rash or itching
• Redness of skin
• Swelling, hives, or blisters
• Signs or symptoms of wound infection
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Storage Instructions
• Store at room temperature.
• Keep the jar tightly sealed.
• Cover with aluminum foil to protect from light.
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