Are we misdiagnosing diabetic foot osteomyelitis? Is the gold standard…gold?

Great work from our long-time SALSAmigos at UTSW in Dallas led by Prof. Larry Lavery.

Abstract:

Are We Misdiagnosing Diabetic Foot Osteomyelitis? Is the Gold Standard Gold? – The Journal of Foot and Ankle Surgery

To compare the incidence of osteomyelitis based on different operational definitions using the gold standard of bone biopsy, we prospectively enrolled 35 consecutive patients who met the criteria of ≥21 years of age and a moderate or severe infection based on the Infectious Diseases Society of America classification. Bone samples were obtained from all patients by percutaneous bone biopsy or intraoperative culture if the patient required surgery. Bone samples were analyzed for conventional culture, histology, and 16S ribosomal RNA genetic sequencing. We evaluated 5 definitions for osteomyelitis: 1) traditional culture, 2) histology, 3) genetic sequencing, 4) traditional culture and histology, and 5) genetic sequencing and histology. There was variability in the incidence of osteomyelitis based on the diagnostic criteria. Traditional cultures identified more cases of osteomyelitis than histology (68.6% versus 45.7%, p​=​.06, odds ratio [OR] 2.59, 95% confidence interval [CI] 0.98 to 6.87), but the difference was not significant. In every case that histology reported osteomyelitis, bone culture was positive using traditional culture or genetic sequencing. The 16S ribosomal RNA testing identified significantly more cases of osteomyelitis compared with histology (82.9% versus 45.7%, p​=​.002, OR 5.74, 95% CI 1.91 to 17.28) and compared with traditional cultures but not significantly (82.9% versus 68.6%, p​=​.17, OR 2.22, 95% CI 0.71 to 6.87). When both histology and traditional culture (68.6%) or histology and genetic sequencing cultures (82.9%) were used to define osteomyelitis, the incidence of osteomyelitis did not change. There is variability in the incidence of osteomyelitis based on how the gold standard of bone biopsy is defined in diabetic foot infections.

One thought on “Are we misdiagnosing diabetic foot osteomyelitis? Is the gold standard…gold?

  1. In order to correctly interpret this data, a STRICT definition of osteomyelitis, based on at least anatomic and volumetric criteria, must be obtained and utilized, then Baysean methodology must be employed utilizing reasonable a priori assumptions.

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